Product Profile

Product Name	Anti-PKR antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	EIF2AK2
Gene Synonyms	PKR PRKR DYT33 LEUDEN EIF2AK1 PPP1R83 Hide
Gene Full Name	eukaryotic translation initiation factor 2 alpha kinase 2
Gene Summary	The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. The encoded protein plays an important role in the innate immune response against multiple DNA and RNA viruses. [provided by RefSeq, Jul 2021] Hide
Molecular Weight(MW)	62 kDa
Cellular Localizatio	Cytoplasm, Nucleus

Application

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WB

Western blot analysis using PKR antibody against HUVEC(1), Hela(2), HEK293(3), K562(4) cell lysate.

·        

IHC

Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using PKR antibody with DAB staining.

·        

ICC/IF

Immunofluorescence analysis of A549 cells using PKR antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.