Product Profile

Product Name	Anti-SUFU antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	SUFU
Gene Synonyms	SUFUH JBTS32 SUFUXL PRO1280 Hide
Gene Full Name	SUFU negative regulator of hedgehog signaling
Gene Summary	The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants. Hide
Molecular Weight(MW)	54 kDa
Cellular Localizatio	Cytoplasm, Nucleus

Application

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WB

Western blot analysis using SUFU antibody against PC-12(1), C2C12(2), HepG2(3) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using SUFU antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of Hela cells using SUFU antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.