Product Profile

Product Name	Anti-EEF1E1 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	EEF1E1
Gene Synonyms	P18 AIMP3
Gene Full Name	eukaryotic translation elongation factor 1 epsilon 1
Gene Summary	This gene encodes a multifunctional protein that localizes to both the cytoplasm and nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage, and it plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream MUTED (muted homolog) gene. An EEF1E1-related pseudogene has been identified on chromosome 2. Hide
Molecular Weight(MW)	20 kDa
Cellular Localizatio	Cytoplasm, Nucleus

Application

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WB

Western blot analysis using EEF1E1 antibody against KATOIII(1),SK-OV-3(2),HEK293(3) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using EEF1E1 antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of MG-63 cells using EEF1E1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.