Product Profile

Product Name	Anti-HNMT antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	HNMT
Gene Synonyms	HMT MRT51 HNMT-S1 HNMT-S2 Hide
Gene Full Name	histamine N-methyltransferase
Gene Summary	In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene. Hide
Molecular Weight(MW)	33 kDa
Cellular Localizatio	Cytoplasm

Application

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WB

Western blot analysis using HNMT antibody against Jurkat(1),COS-7(2),MCF-7(3),A431(4) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded breast cancer tissues using HNMT antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of Hela cells using HNMT antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.