Product Profile

Product Name	Anti-14-3-3 Theta antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFWB
	WB:1:200-1:2000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	YWHAQ
Gene Synonyms	1C5 HS1 14-3-3
Gene Full Name	tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta
Gene Summary	This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. Hide
Molecular Weight(MW)	28 kDa
Cellular Localizatio	Cytoplasm

Application

·        

WB

Western blot analysis using 14-3-3 Theta antibody against HepG2(1),Raji(2),Hela (3),A549(4)cell lysate.

·        

ICC/IF

Immunofluorescence analysis of A549 cells using 14-3-3 Theta antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.