Product Profile

Product Name	Anti-P4HB antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	P4HB
Gene Synonyms	DSI GIT PDI PHDB PDIA1 PO4DB PO4HB PROHB CLCRP1 ERBA2L P4Hbeta More
Gene Full Name	prolyl 4-hydroxylase subunit beta
Gene Summary	This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. More
Molecular Weight(MW)	57 kDa
Cellular Localizatio	Cell membrane, Endoplasmic reticulum, Membrane

Application

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WB

Western blot analysis using P4HB antibody against PANC-1(1),COS7(2),MCF-7(3),NIH3T3(4)cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using P4HB antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of CAL-27 cells using P4HB antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.