Product Profile

Product Name	Anti-AKAP13 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHC
	IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	AKAP13
Gene Synonyms	BRX LBC p47 HA-3 Ht31 c-lbc PRKA13 AKAP-13 AKAP-Lbc ARHGEF13 PROTO-LB PROTO-LBC More
Gene Full Name	A-kinase anchoring protein 13
Gene Summary	The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms containing c-terminal dbl oncogene homology (DH) and pleckstrin homology (PH) domains. The DH domain is associated with guanine nucleotide exchange activation for the Rho/Rac family of small GTP binding proteins, resulting in the conversion of the inactive GTPase to the active form capable of transducing signals. The PH domain has multiple functions. Therefore, these isoforms function as scaffolding proteins to coordinate a Rho signaling pathway, function as protein kinase A-anchoring proteins and, in addition, enhance ligand-dependent activity of estrogen receptors alpha and beta. More
Molecular Weight(MW)	307 kDa
Cellular Localizatio	Cytoplasm, Membrane, Nucleus

Application

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IHC

Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using AKAP13 antibody with DAB staining.

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IHC

Immunohistochemical analysis of paraffin-embedded breast cancer tissues using AKAP13 antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of A549 cells using AKAP13 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.