Product Profile

Product Name	Anti-NEDD9 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHC
	IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	NEDD9
Gene Synonyms	CAS2 CASL HEF1 CAS-L CASS2 Hide
Gene Full Name	neural precursor cell expressed, developmentally down-regulated 9
Gene Summary	The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants. Hide
Molecular Weight(MW)	93 kDa
Cellular Localizatio	Cell junction, Cell projection, Cytoplasm, Cytoskeleton, Golgi apparatus, Nucleus

Application

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IHC

Immunohistochemical analysis of paraffin-embedded breast cancer tissues using NEDD9 antibody with DAB staining.

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IHC

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using NEDD9 antibody with DAB staining.

        

ICC/IF

Immunofluorescence analysis of A549 cells using NEDD9 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.