Product Profile

Product Name	Anti-GOT2 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	GOT2
Gene Synonyms	KAT4 DEE82 KATIV KYAT4 mitAAT More
Gene Full Name	glutamic-oxaloacetic transaminase 2
Gene Summary	Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene. More
Molecular Weight(MW)	48 kDa
Cellular Localizatio	Cell membrane, Membrane, Mitochondrion

Application

·        

WB

Western blot analysis using GOT2 antibody against Jurkat(1),NIH3T3(2), A431(3)cell lysate.

·        

IHC

Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using GOT2 antibody with DAB staining.

·       

ICC/IF

Immunofluorescence analysis of T24 cells using GOT2 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.