Product Profile

Product Name	Anti-CD247 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	CD247
Gene Synonyms	T3Z CD3H CD3Q CD3Z TCRZ IMD25 CD3-ZETA Hide
Gene Full Name	CD247 molecule
Gene Summary	The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Hide
Molecular Weight(MW)	19 kDa（Observed:24 kDa）
Cellular Localizatio	Membrane

Application

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WB

Western blot analysis using CD247 antibody against A431(1), HepG2(2) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded breast cancer tissues using CD247 antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of CAL-27 cells using CD247 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.