Product Profile

Product Name	Anti-CRKL antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:2000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1mg/ml
Purification	Protein A

Target Information

Gene Symbol	CRKL
Gene Full Name	CRK like proto-oncogene, adaptor protein
Gene Summary	This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009] Hide
Molecular Weight(MW)	34kD（Observed 50 kD）
Cellular Localizatio	Cytosol; Nucleus; Plasma Membrane

Application

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WB

Western blot analysis using CRKL antibody against HepG2(1) cell lysate.

·        

IHC

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using CRKL antibody with DAB staining.

·       

ICC/IF

Immunofluorescence analysis of Hela cells using CRKL antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:2000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.