Product Profile

Product Name	Anti-EIF4E antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:2000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1mg/ml
Purification	Protein A

Target Information

Gene Symbol	EIF4E
Gene Synonyms	CBP EIF4F AUTS19 EIF4E1 eIF-4E EIF4EL1 More
Gene Full Name	eukaryotic translation initiation factor 4E
Gene Summary	The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] More
Molecular Weight(MW)	25KD
Cellular Localizatio	Cytoplasm, Nucleus

Application

        

WB

Western blot analysis using EIF4E antibody against Hek293(1) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using EIF4E antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of A549 cells using EIF4E antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:2000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.