Product Profile

Product Name	Anti-CD73 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:2000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1mg/ml
Purification	Protein A

Target Information

Gene Symbol	NT5E
Gene Synonyms	NT eN NT5 NTE eNT CD73 E5NT CALJA Hide
Gene Full Name	5'-nucleotidase ecto
Gene Summary	The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011] Hide
Molecular Weight(MW)	63kD(Observed:54kD）
Cellular Localizatio	Cell membrane

Application

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WB

Western blot analysis using CD73 antibody against A431(1) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using CD73 antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of MG-63 cells using CD73 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:2000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.