Product Profile

Product Name	Anti-BMI1 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:2000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1mg/ml
Purification	Protein A

Target Information

Gene Symbol	BMI1
Gene Synonyms	PCGF4 RNF51 FLVI2/BMI1 flvi-2/bmi-1 Hide
Gene Full Name	BMI1 proto-oncogene, polycomb ring finger
Gene Summary	This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene. [provided by RefSeq, Sep 2015] Hide
Molecular Weight(MW)	37KD
Cellular Localizatio	Cytoplasm, Nucleus

Application

·       

WB

Western blot analysis using BMI1 antibody against HepG2(1), A431(2) cell lysate.

·        

IHC

Immunohistochemical analysis of paraffin-embedded liver cancer tissues using BMI1 antibody with DAB staining.

·        

ICC/IF

Immunofluorescence analysis of NIH3T3 cells using BMI1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:2000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.